Total RNA extraction is a fundamental step in numerous molecular biology experiments. This process requires the careful recovery of total RNA from diverse biological matrices, such as tissues, cells, and body fluids. The goal is to obtain high-quality RNA that is free from contaminating substances. A variety of techniques are available for total R

RNA is a fragile molecule that is easily degraded by enzymes called RNases. Therefore, it is essential to precisely handle RNA samples and use clean techniques during isolation and purification processes. There are several methods available for isolating RNA from various sources, including {phenol-chloroform extraction, column chromatography, and m